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I've generated a CA and multiple certificates (signed by CA) using OpenSSL and I have a .NET/C# client and server both using SslStream which each have their own certificates/keys, mutual authentication is enabled and revocation is disabled.

I'm using RemoteCertificateValidationCallback for SslStream to validate the remote server's certificate and I was hoping I could just load the CA's public certificate (as a file) in the program and use it to verify the remote certificate rather then actually installing the CA in the Windows Certificate Store. The problem is the X509Chain won't show anything else unless I install the CA into the store, either will the Windows CryptoAPI shell when I open a PEM version of one of the certificates.

My question is, how can I verify a certificate has been signed by my specific CA just by using the CA's public certificate file without using Windows certificate store or WCF when RemoteCertificateValidationCallback, X509Certificate and X509Chain don't seem to give me anything to work with?

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Because the CA certificate is NOT in the root certificate store, you will have within the RemoteCertificateValidationCallback() an error flag of SslPolicyErrors.RemoteCertificateChainErrors ; a possibility is to validate explicitely the certificate chain against your own X509Certificate2Collection, since you are not using the local store.

if (sslPolicyErrors == SslPolicyErrors.RemoteCertificateChainErrors)
{
    X509Chain chain0 = new X509Chain();
    chain0.ChainPolicy.RevocationMode = X509RevocationMode.NoCheck;
    // add all your extra certificate chain
    chain0.ChainPolicy.ExtraStore.Add(new X509Certificate2(PublicResource.my_ca));
    chain0.ChainPolicy.VerificationFlags = X509VerificationFlags.AllowUnknownCertificateAuthority;
    isValid = chain0.Build((X509Certificate2)certificate);
}

You can also re-use the chain passed in the callback, add your extra certificate(s) in the ExtraStore collection, and validate with the AllowUnknownCertificateAuthority flag which is needed since you add untrusted certificate(s) to the chain.

You could also prevent the original error by adding programmatically the CA certificate in the trusted root store (of course it opens a popup, for it is a major security problem to globally add a new trusted CA root) :

var store = new X509Store(StoreName.Root, StoreLocation.CurrentUser);
store.Open(OpenFlags.ReadWrite);
X509Certificate2 ca_cert = new X509Certificate2(PublicResource.my_ca);
store.Add(ca_cert);
store.Close();

EDIT: For those who want to clearly test the chain with your CA :

Another possibility is to use the library BouncyCastle to build the certificate chain and validate the trust. The options are clear and errors are easy to understand. In cas of success it will build the chain, otherwise an exception is returned. Sample below :

// rootCerts : collection of CA
// currentCertificate : the one you want to test
var builderParams = new PkixBuilderParameters(rootCerts, 
                        new X509CertStoreSelector { Certificate = currentCertificate });
// crls : The certificate revocation list
builderParams.IsRevocationEnabled = crls.Count != 0;
// validationDate : probably "now"
builderParams.Date = new DateTimeObject(validationDate);

// The indermediate certs are items necessary to create the certificate chain
builderParams.AddStore(X509StoreFactory.Create("Certificate/Collection", new X509CollectionStoreParameters(intermediateCerts)));
builderParams.AddStore(X509StoreFactory.Create("CRL/Collection", new X509CollectionStoreParameters(crls)));

try
{
    PkixCertPathBuilderResult result = builder.Build(builderParams);
    return result.CertPath.Certificates.Cast<X509Certificate>();
    ...

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